E3S Web Conf.
Volume 52, 2018CSSPO International Conference 2018: Towards Inclusive & Sustainable Agriculture – Harmonizing Environmental, Social and Economic Dimensions: Is it Possible?
|Number of page(s)||10|
|Published online||27 August 2018|
Microsatellite analysis of genetic diversity and structure of Bruguiera gymnorrhiza and Kandelia obovate
Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara,
Jl. Tri Dharma Ujung No. 1 Medan, North Sumatera 20155,
2 Mangrove and Bio-Resources Group, Center of Excellence for Natural Resources Based Technology, Universitas Sumatera Utara, Medan North Sumatera 20155, Indonesia
3 International Society for Mangrove Ecosystems, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan
4 Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan
* Corresponding Author: firstname.lastname@example.org
Microsatellite loci were used for estimating genetic diversity and structure for three populations of B. gymnorrhiza and K. obovata (Rhizophoracea) in Okinawa, Japan. Thirty propagules of individual samples representing the population of both species were genotyped at five microsatellites. The level of observed heterozygosity (HO) was observed for several population, overall loci, ranged 0.422-0.800 with an average 0.627 for B. gymnorrhiza and 0.477-0.822 with an average 0.665 for K. obovata, indicating both species had relatively low genetic diversity. Both species showed low levels of allelic diversity, 3-5 and 3-5 alleles per locus, respectively. Gene diversitywas also maintained within populations (HS: 0.741 and 0.954). Additionally, an analysis of molecular variance (AMOVA) based on the immeasurable alleles model (F-statistics), for B. gymnorrhiza and K. obovata found that most of the variation resided within individuals in the total populations, i.e. 79.78 % and 69.90 % respectively, and among individuals within populations, i.e.14.30 % and 27.95 % respectively. There was little variation between populations, i.e. 5.92 % and 2.15 % for B. gymnorrhiza and K. obovata, respectively. The high-level genetic differentiation within individuals and populations both species may be due to the geographic range of the species, mating system, and environmental factors.
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