Removal of Iron using Isolated Bacteria (Vibrio alginolyticus) in seawater at Ship Dismantling Area

¹ Department of Environmental Engineering, Faculty of Civil, Environmental and Geo Engineering, Sepuluh November Institute of Technology, Keputih, Sukolilo, 60111 Surabaya, Indonesia
² Department of Ocean Engineering, Faculty of Marine Technology, Sepuluh November Institute of Technology, Keputih, Sukolilo, 60111 Surabaya, Indonesia

^* Corresponding author: harminsulis@gmail.com , harmin_st@its.ac.id

Abstract

The ship dismantling activity can cause heavy metal pollution in the environment. One of technology to remediate is bioremediation using bioaugmentation and/or biostimulation methods. The purpose of this research was to determine the removal of iron by Vibrio alginolyticus and nutrient addition. Vibrio alginolyticus was halophilic bacteria that isolated from seawater at this area. The laboratory test was conducted using size erlenmeyer of 250 mL. The 5% (v/v) of Vibrio alginolyticus suspension was added in each reactor. After that, the nutrient with ratio of C : N : P (100 : 10 : 1) also was added in each reactor. The removal test was carried out for 14 days. Concentration of iron was analyzed using Atomic Absorption Spectrophotometers (AAS). Based on the results, the percentage of iron removal at location 1 with treatment was 94.9 ± 3.6%. It showed a higher value than compared to iron removal at control 1 which was only 50.4±1.8 %. Whereas at location 2, the percentage of iron removal with treatment reached 53.7±13.1% and it was only 16.7±13.1% at control 2. This indicated that the treatment of the addition of Vibrio alginolyticus and nutrients can improve the iron remediation process.