| Issue |
E3S Web Conf.
Volume 131, 2019
2nd International Conference on Biofilms (ChinaBiofilms 2019)
|
|
|---|---|---|
| Article Number | 01023 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/e3sconf/201913101023 | |
| Published online | 19 November 2019 | |
Amplification Techniques of Recombinase & Polymerase and their Application in Parasite Detection
Shanxi Medical University, Shanxi Taiyuan 030001
To study the application principles of recombinase polymerase amplification (RPA) and the specific situations of detecting parasites, the principles of RPA are analyzed to find the optimal temperature conditions, advantages, and disadvantages. Then, the parasites are detected to observe the application characteristics of the RPA method. The results show that RPA is a kind of novel isothermal nucleic acid amplification technology, which is an open detection method. It has high sensitivity and specificity when being operated at 37-42°C, which makes it very suitable for early detection of pathogen infection. Besides, it also has high sensitivity and specificity in parasite detection. Therefore, the RPA technology has better performances and excellent applications in parasite detection, which has a certain significance for the future application of the technology in more fields.
© The Authors, published by EDP Sciences, 2019
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