| Issue |
E3S Web Conf.
Volume 145, 2020
2019 International Academic Exchange Conference on Science and Technology Innovation (IAECST 2019)
|
|
|---|---|---|
| Article Number | 01002 | |
| Number of page(s) | 4 | |
| Section | International Conference on Biotechnology and Food Science | |
| DOI | https://doi.org/10.1051/e3sconf/202014501002 | |
| Published online | 06 February 2020 | |
Preliminary researches on conditions to extract alchol soluble proteins of leaves of Wedelia trilobata (L.) Hitchc.
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
* Corresponding author is Gan Zhao whose E-mail is zg200010@163.com.
The conditions to extract alchol soluble proteins of leaves of Wedelia trilobata (L.) Hitchc. were discussed. When the extractant was alcohol, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10, temperature for extraction was 50°Cand the extraction period was 2hrs, the concentration of alcohol solution needed for the best extraction effect was 95% (V/V). When the extractant was 75% alcohol solution, the temperature for extraction was 50°Cand the extraction period was 2hrs, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio needed for the best extraction effect was 1:20. When the extractant was 75% alcohol solution, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10 and the extraction period was 2 hrs, the temperature needed for the best extraction effect was 55°C. When the extractant was 75% alcohol solution, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10 and the temperature for extraction was 50°C, the extraction period needed for the best extraction effect was 2hrs.
© The Authors, published by EDP Sciences 2020
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