E3S Web Conf.
Volume 151, 2020The 1st International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2019)
|Number of page(s)||4|
|Published online||14 February 2020|
Oviposition Deterrent Activity and Ovicidal Effect of Pometia pinnata Leaves Extract against Dengue Vector, Aedes aegypti (Diftera: Culicidae)
Student of Faculty of Medicine, Universitas Islam Indonesia
2 Departement of Parasitology, Faculty of Medicine, Universitas Islam Indonesia
* Corresponding author: firstname.lastname@example.org
Aedes aegypti is the main vector of dengue fever that most commonly occurred in Indonesia. The application of botanical insecticides is being developed as an alternative vector control. Botanical components have been reported as the potential alternative to inhibit the process of oviposition and fecundity suppression. This study aims to determine the oviposition deterrent activity and ovicidal effects of ethanol extract of Pometia pinnata leaves against Aedes aegypti. Oviposition deterrent assay was conducted on twenty bloodfed females in a cage (40 cm x 40 cm x 40 cm). One treated oviposition trap and one control oviposition trap were placed in the opposite corner of the cage. Four replicates were performed for each concentration. The eggs were counted and analyzed after 72 hours of trial. Ethanol extract of Pometia pinnata leaves have antioviposition effect on female mosquitoes Aedes aegypti at 400 ppm (effective repellency / ER = 56.38%; oviposition active index/OAI = -0.39) and 500 ppm (ER = 66.4%; OAI = -0.49). In the ovicidal assay, twentyfive eggs are used for determination ovicidal effect and five replicates were performed for each concentration. The hatched larvae were collected and counted daily. The ethanolic extract of Pometia pinnata leaves at 0.05%, 0,1%, 0.15%, 0.2%, and 0.25% concentration have ovicidal activity of 11%, 28%, 87%, 94%, and 98% against Aedes aegypti eggs, respectively. This study revealed that the ethanol extract of Pometia pinnata leaves could be a potential alternative in controlling the dengue vector.
Key words: oviposition / ovicidal / dengue / Aedes aegypti / Pometia pinnata
© The Authors, published by EDP Sciences, 2020
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