Issue |
E3S Web Conf.
Volume 151, 2020
The 1st International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2019)
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Article Number | 01031 | |
Number of page(s) | 4 | |
DOI | https://doi.org/10.1051/e3sconf/202015101031 | |
Published online | 14 February 2020 |
Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat
Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
* Corresponding author: monaairin@ugm.ac.id
Progesterone (P4) is a dominant hormone during pregnancy. In the later stage of pregnancy, the stress hormone particularly cortisol (C) may increase for initiating the parturition process as a consequence of fetal stress. This study was a preliminary study to compare the concentration of P4 and C in feces of Etawah Crossbreed Goat during their last stage of pregnancy and post partus. This study used 5 pregnant Etawah Crossbreed Goats (t 20th weeks) of pregnancy. Fecal samples were collected in the 20th week of pregnancy to 2 weeks of postpartum. All fecal samples were then dried using a freeze dryer (Labfreez FD10-MR) for 7 days at -80°C. Afterward, dried feces were pulverized and extracted by using 3ml of methanol 80%. The fecal extract was then analyzed the P4 and C concentrations using the enzyme-linked immunosorbent assay method. Concentrations of P4 and C metabolites in the last stage of pregnancy were 5,506.18 3,396.72 ng/g dry feces and 136,625.83 42,479.22 ng/g feces, respectively. Concentrations of P4 and C metabolites in the 2 weeks postpartum decreased at 669.38 P 643.9 ng/g feces and 110,295 / 14,378, 8 ng/g feces, respectively. It canbe concluded that there was a difference in the fecal progesterone and cortisol concentrations between the last phase of pregnancy and the postpartum phase.
Key words: crossbreed / feces / metabolite hormone / cortisol / progesterone
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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