E3S Web Conf.
Volume 165, 20202020 2nd International Conference on Civil Architecture and Energy Science (CAES 2020)
|Number of page(s)||4|
|Section||Chemical Engineering and Materials Science|
|Published online||01 May 2020|
Study on chemical constituents of different species of Dendrobium
School of Ecology Technology and Engineering, 201418 Shanghai Institute of Technology, Shanghai China
* Corresponding author: email@example.com
The objective of this study is to determine the erianin of 10 species of Dendrobium by high performance liquid chromatography (HPLC). Processing techniques were washed, dried and ground. The research method for determining the content of erianin was adopted from Chinese Pharmacopoeia 2015 Edition. Acetonitrile-0.05% phosphoric acid (37:63) was used as mobile phase. The samples were separated on Sharpsil-TC18 column(4.6*150mm;5μm) at a flow rate of 1.2mL/min and detected at 230nm, and the column temperature was kept at 30℃. The injection volume was 20μL. The number of theoretical plates was not less than 6000 according to the chromatographic peak of erianin. The result of the study showed erianin was detected only in Dendrobium chrysotoxum among the 10 species of Dendrobium. and the content was 0.098%. Conclusion: The content of erianin in Dendrobium chrysotoxum met the specification of no less than 0.03% in the 2015 edition of Chinese Pharmacopoeia.
© The Authors, published by EDP Sciences, 2020
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