Issue |
E3S Web Conf.
Volume 185, 2020
2020 International Conference on Energy, Environment and Bioengineering (ICEEB 2020)
|
|
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Article Number | 04007 | |
Number of page(s) | 5 | |
Section | Chemical Engineering and Food Biotechnology | |
DOI | https://doi.org/10.1051/e3sconf/202018504007 | |
Published online | 01 September 2020 |
Responses to Higher Concentration Lecithin of Apoptosis Relevant Factors and Detection of Specific Lecithin Binding Proteins by AC-MS assay
College of Life Sciences, South China Agricultural University, Guangzhou, 510614, China
* Corresponding Author’s E-mail: yxyu@scau.edu.stu.cn
Lecithin in mixed micelles attenuates cytotoxicity by interacting with proteins or other cell inclusions. The reaction was then captured via magnetic beads binding approach. This research proposal was designed to discover the impacts of lecithin on apoptosis relevant factors (including caspase 3/8/9 and PARP) by Western Blot and specific protein binding mechanism of lecithin detoxification pathway, using caco-2 cell model. Preliminary investigation of the properties of the proteins was conducted by MS (Mass Spectrometry) couplet to AC (Affinity Chromatography) assay. Further research of particular proteins was to identify the size and peptide sequence. This study also provided solid evidence for the previous research result, which was lecithin reduce cytotoxicity induced by SDC contained mixed micelles.
© The Authors, published by EDP Sciences, 2020
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