| Issue |
E3S Web of Conf.
Volume 222, 2020
International Scientific and Practical Conference “Development of the Agro-lndustrial Complex in the Context of Robotization and Digitalization of Production in Russia and Abroad” (DAIC 2020)
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|---|---|---|
| Article Number | 02047 | |
| Number of page(s) | 5 | |
| Section | Modern Directions of Highly Productive Environmentally Friendly Agro- and Water Management, Development and Implementation of Systems for the Rational use of Modern Chemical and Biological Protection of Agricultural Plants and Animals, the Use of New Drugs, Growth Agents, Antidepressants, Immunostimulants, Probiotics and Feed Additives | |
| DOI | https://doi.org/10.1051/e3sconf/202022202047 | |
| Published online | 22 December 2020 | |
Betaine biosynthesis in a heterologous expression system based on the B12 producer Pseudomonas denitrificans
1 Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia
2 G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia
3 Far Eastern Federal University, 690922 Vladivostok, Russia
* Corresponding author: yn80@mail.ru
Glycine betaine is an important donor of methyl groups in various metabolic processes of the cell and acts as an osmoprotector when exposed to various abiotic stresses in pro- and eukaryotic organisms. Moreover, exogenous application of betaine activates the production of vitamin B12 in industrial strains-producers. In this work, we have developed a new technology for microbiological betaine synthesis that can be used in biotechnology to activate B12 biosynthesis during large-scale fermentation of Pseudomonas denitrificans.
© The Authors, published by EDP Sciences, 2020
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