| Issue |
E3S Web Conf.
Volume 224, 2020
Topical Problems of Agriculture, Civil and Environmental Engineering (TPACEE 2020)
|
|
|---|---|---|
| Article Number | 02005 | |
| Number of page(s) | 10 | |
| Section | Information Technologies for Environmental Sustainability | |
| DOI | https://doi.org/10.1051/e3sconf/202022402005 | |
| Published online | 23 December 2020 | |
Possibilities of using a miniature fluorescence microscope
1
Peter The Great St. Petersburg Polytechnic University, St. Petersburg, Russian Federation
2
UT Southwestern Medical Center at Dallas, Dallas, TX, USA
* Corresponding author: marina.bolsunovskaia@spbpu.com
The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment’s data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.