E3S Web Conf.
Volume 224, 2020Topical Problems of Agriculture, Civil and Environmental Engineering (TPACEE 2020)
|Number of page(s)||10|
|Section||Agriculture and Bioscience|
|Published online||23 December 2020|
In vitro modelling of salinity stress for the selection of stress-tolerant birch lines
All-Russian Research Institute of Forest Genetics, Breeding and Biotechnology, Lomonosova Street, 105, 394087, Voronezh, Russia
2 Voronezh State University, Universitetskaya Square 1, 394018, Voronezh, Russia
* Corresponding author: email@example.com
This paper examines the salinity stress effect in vitro culture of microshoots of 10 birch clones: Betula pendula L., B. pendula Roth var. carelica (Mercklin) Hämet-Ahti, B. pendula f. ’dalecarlica’ (L.f.) Schneid., B. pubescens Ehrh. We used economically valuable and genetically diverse (various species, varieties, hybrids, polyploids) birch material from in vitro clone collection. We tested 3 in vitro selection methods on 1/2 MS hormone-free nutrient medium: mild – with a prolonged exposure to 0.2% NaCl; gradual – with a step-by-step increase in osmotic concentration (from 0.2% to 1%); severe – with a sublethal (1%) NaCl concentration. We established that the gradual method of NaCl exposure according to the following pattern: 0.2% → 0% → 0.5% → 0% → 0.75% → 0% → 1.0% resulted in the best selective effect for birch, as it provides a clear differentiation of clones in terms of their salinity tolerance. We identified that the genotype had a significant impact on the preservation of the explants and their ability to regenerate under salinity stress. The study showed that tetraploid clones of downy birch and a triploid clone of Karelian birch of a pronounced mixoploid nature had the greatest salinity tolerance.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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