E3S Web Conf.
Volume 224, 2020Topical Problems of Agriculture, Civil and Environmental Engineering (TPACEE 2020)
|Number of page(s)||6|
|Section||Agriculture and Bioscience|
|Published online||23 December 2020|
Effect of silicon chelates from plant raw materials on some physiological parameters in strawberry under in vitro conditions
Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, st. Zolotodolinskaya, 101, 630090, Russian Federation
2 Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of the Russian Academy of Sciences, st. Kutateladze, 18, Novosibirsk, 630128, Russian Federation
* Corresponding author: email@example.com
For the first time, the effect mechanocomposite based on biogenic silica and green tea flavonoids (MC) on reduction of oxidative stress in microshoots of strawberry plantlets (cv. “Solnechnaya polyanka”) during in vitro multiplication, shoot elongation and rooting stages were established. Compared with the plantlets cultured in MC-free media, the ones cultured in media supplemented with MC showed increased key antioxidant enzyme activities (superoxide dismutase – SOD, peroxidase – POD, catalase – CAT). SOD activity increased from 1.1 to 1.4 times at 2.5 and 5.0 mg L-1 MC, POD activity – from 1.8 to 3.8 times at 5.0 and 10.0 mg L-1 MC, and CAT activity – from 1.1 to 1.4 times at 5.0 mg L-1 MC depending on the stage of micropropagation. The high antioxidant enzyme activities correlated with the decrease of H2O2 content (up to 1.7 times compared with the control). The revealed changes in the physiological status of strawberry plantlets in the presence of MC contribute to the further successful acclimatization of regenerants to ex vitro conditions. Thus, these results have demonstrated the potential of “green chemistry” for the production of healthy plant material of strawberry cultivars.
© The Authors, published by EDP Sciences, 2020
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