| Issue |
E3S Web Conf.
Volume 224, 2020
Topical Problems of Agriculture, Civil and Environmental Engineering (TPACEE 2020)
|
|
|---|---|---|
| Article Number | 04022 | |
| Number of page(s) | 8 | |
| Section | Agriculture and Bioscience | |
| DOI | https://doi.org/10.1051/e3sconf/202022404022 | |
| Published online | 23 December 2020 | |
Editing of the MYB genes in Brassica napus as a method to increase anthocyanin pigmentation and stress tolerance
Institute of biochemistry and genetics UFRC RAS, October Avenue, 71, 450054, Ufa, Russia
* Corresponding author: mikhele@list.ru
Anthocyanin hyperaccumulation is an important agricultural trait, associated with resistance to abiotic stress, pests, phytopathogenic fungi and bacterial diseases. B. napus with increased anthocyanin pigmentation can be generated by genome editing. Many transcription factors of the MYB family are involved in stress response and anthocyanin biosynthesis. Genes AtMYB60, AtCPC and AtMYBL2 are negative regulators of anthocyanin biosynthesis in Arabidopsis, therefore the knockout of these genes can result in increased anthocyanin pigmentation. gRNA spacers were synthesized to target the orthologs of these genes, identified in Brassica napus. Resulting genetic constructs were introduced to the plant tissues by agroinfiltration. Transient expression of gRNAs targeting DNA-binding domains of MYB transcription factors along with Cas9 nuclease successfully promoted anthocyanin hyperaccumulation. These genetic constructs can be used for genome editing and production of new colored and stress tolerant varieties of oilseed rape.
© The Authors, published by EDP Sciences, 2020
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