E3S Web Conf.
Volume 226, 2021The 1st International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology (ICoN BEAT 2019)
|Number of page(s)||7|
|Published online||05 January 2021|
Vinasse as Cultivation Medium of Chlorella sp. to Produce Poly–Hydroxy Butyrate in Various Limited Low–Cost Primary Nutrient
1 Department of Chemical Engineering, Faculty of Engineering, Setia Budi University, Jl. Letjend Sutoyo Mojosongo, Solo 57127, Central Java, Indonesia
2 Department of Chemical Analyst, Faculty of Engineering, Setia Budi University, Jl. Letjend Sutoyo Mojosongo, Solo 57127, Central Java, Indonesia
3 Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Jl. Prof. Soedarto, Tembalang, Semarang 50275, Central Java, Indonesia
4 Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Universitätsring 1, 1010 Wien, Austria
* Corresponding author: email@example.com
Vinasse is ethanol wastewater that still contains nutrients. It can be medium cultivation for Chlorella sp. Cultivation Chlorella sp. in vinasse did not only minimize its COD content but also potentially produce Poly–Hydroxy Butyrate (PHB) in a limited nutrient. This paper presents a cultivation process of Chlorella sp. in vinasse on the various limited nutrient. Chlorella sp. was cultivated in vinasse by adding complete nutrient (urea and TSP), TSP (limited N), and urea (limited P). Experimental data was optimized by a mathematical model to predict the behavior of the Chlorella sp. in limited nutrient systematically. The study confirmed that the best condition of the cultivation medium of Chlorella sp. to minimize COD content in vinasse by addition phosphate into the reactor. However, PHB could be best accumulated in the cell on limited phosphate.
Key words: Biological plastic / cultivation medium / environment friendly / stillage / wastewater utilization
© The Authors, published by EDP Sciences, 2021
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