Entrapment Formulation for env-Tm Gene Based on Chitosan Low Molecular Weight as a Jembrana Disease Virus Vaccine Candidate

¹ Department of Biotechnology, Postgraduate School, Universitas Gadjah Mada, Jl. Teknika Utara, Sleman, Yogyakarta 55281, Indonesia
² Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna Karangmalang No.2, Yogyakarta 55281, Indonesia

^* Corresponding author: asmaranikusumawati2018@gmail.com

Abstract

Jembrana disease (JD) caused by Jembrana Disease Virus (JDV) becomes an obstacle in Bali cattle (Bos javanicus). The development of JD vaccines has a critical meaning to prevent losses in the B. javanicus in Indonesia and is one of the models in the development of the Human Immunodeficiency Virus (HIV) vaccine. The development of vaccines for JDV has carried out DNA vaccines that are expected to provide better immunological effects. This study aimed to determine the low molecular weight chitosan (LC) entrapment towards pEGFP-C1-env-Tm in the formation of Chitosan Nanoparticles Low/pEGFP-C1-env-Tm complex. The env-Tm gene was inserted in pEGFP-C1 into the pEGFPC1/ env-Tm construct transformed on the E. coli DH5α host. The construct was formulated into LC/pEGFP-C1/env-Tm complex with a low molecular weight chitosan concentration of 0.06 % and the ratio of pEGFP-C1/env-Tm: LC (wt/wt) was 1:0.5-1:3. The complexes were then analyzed by gel retardation assay agarose 1 %. The results of this study indicated that the best entrapment results of low molecular weight chitosan to pEGFPC1/ env-Tm was in the mass ratio of pEGFP-C1/env-Tm: LC was 1:2. The best formulation entrapment for env-Tm by low molecular weight chitosan 0.06 % is in the rate 1:2.