E3S Web Conf.
Volume 226, 2021The 1st International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology (ICoN BEAT 2019)
|Number of page(s)||4|
|Published online||05 January 2021|
Entrapment Formulation for env-Tm Gene Based on Chitosan Low Molecular Weight as a Jembrana Disease Virus Vaccine Candidate
1 Department of Biotechnology, Postgraduate School, Universitas Gadjah Mada, Jl. Teknika Utara, Sleman, Yogyakarta 55281, Indonesia
2 Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna Karangmalang No.2, Yogyakarta 55281, Indonesia
* Corresponding author: email@example.com
Jembrana disease (JD) caused by Jembrana Disease Virus (JDV) becomes an obstacle in Bali cattle (Bos javanicus). The development of JD vaccines has a critical meaning to prevent losses in the B. javanicus in Indonesia and is one of the models in the development of the Human Immunodeficiency Virus (HIV) vaccine. The development of vaccines for JDV has carried out DNA vaccines that are expected to provide better immunological effects. This study aimed to determine the low molecular weight chitosan (LC) entrapment towards pEGFP-C1-env-Tm in the formation of Chitosan Nanoparticles Low/pEGFP-C1-env-Tm complex. The env-Tm gene was inserted in pEGFP-C1 into the pEGFPC1/ env-Tm construct transformed on the E. coli DH5α host. The construct was formulated into LC/pEGFP-C1/env-Tm complex with a low molecular weight chitosan concentration of 0.06 % and the ratio of pEGFP-C1/env-Tm: LC (wt/wt) was 1:0.5-1:3. The complexes were then analyzed by gel retardation assay agarose 1 %. The results of this study indicated that the best entrapment results of low molecular weight chitosan to pEGFPC1/ env-Tm was in the mass ratio of pEGFP-C1/env-Tm: LC was 1:2. The best formulation entrapment for env-Tm by low molecular weight chitosan 0.06 % is in the rate 1:2.
Key words: Env-tm gene / jembrana disease virus / low molecular
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.