Lignin and Cellulose Content of Fermented Rice Straw with Aspergillus niger (van Tieghem) and Trichoderma mutan AA1

¹ Department of Animal Husbandry, Faculty of Agriculture, Veteran Bangun Nusantara University, Jl. Letjen Sujono Humardani No. 1, Sukoharjo 57521, Central Java, Indonesia
² Department of Agribusiness, Faculty of Agriculture, Veteran Bangun Nusantara University, Jl. Letjen Sujono Humardani No. 1, Sukoharjo 57521, Central Java, Indonesia
³ Department of Environmental Science, University of Latvia, Jelgavas Street 1, Room 302, Riga LV-1004, Latvia
⁴ Department of Agriculture, Faculty of Animal Science and Agriculture, Diponegoro University, Jl. Prof. Sudarto No.13, Tembalang, Semarang 50275, Central Java, Indonesia

^* Corresponding author: srisukaryani@gmail.com

Abstract

The rice straw has potential to be used as an alternative ruminant feed. However, it has limiting factors i.e low crude protein, high crude fiber, lignin, cellulose, and silica content. To overcome the limiting factors, immersion in a solution of alkaline (lime) or fermentation by using inoculum microbial cellulolytic and lignocellulolytic (Trichoderma mutan AA1 and Aspergillus niger.). The research method was experimental, with four treatments and repeated five times. Completely randomized design was used and if there are differences among treatments a further test with DMRT was carried out (level 1 % and 5 %). These treatments were T0: The rice straw without t fermentation; T1: Fermented with A. niger; T2: Fermented with T. mutan AA1; T3: Fermented with a combination A. niger and T. mutan AA1. The results showed that the rice straw fermented with A. niger and T. mutants AA1 very significantly increased the cellulose and decreased lignin content. The highest cellulose reached on T3 (20.297 %) followed by T2 (18.191 %), T1 (17.712 %) and T0 (16.747 %), respectively. While the lowest content of lignin reached on T3 (14.793 %), followed T2 (26.063 %), T1 (26.421 %) and T0 (38.164 %).