The Vegetative Growth Response of Detam Soybean Varieties towards Bacillus subtilis and Trichoderma sp. Applications as Bio-fertilizer

Department of Agrotechnology, Faculty of Science and Technology, Universitas Muhammadiyah Sidoarjo

^* Corresponding author: sutarman@umsida.ac.id

Abstract

This study aims to determine the effect of bacterial isolates of Bacillus subtilis Bs-Sdj-01 and Trichoderma sp. Tc-Jro-02 isolates as biological fertilizer in plant growth until the end of the vegetative phase. The experiment was arranged factorially in a Completely Randomized Design (CRD) with each factor being the application of B. subtilis consisting of and without B. subtilis and the Trichoderma application consisting of with and without Trichoderma; the experiment was repeated four times. The observed variables were: plant height, stem diameter, number of leaves, leaf area, stover dry weight, root dry weight, and B. subtilis population at the end of the observation. Data were analyzed using with ANOVA and HSD tests at the 5% level. The combination of Trichoderma sp. and B. subtilis bacteria produce the highest increase in plant height, stem diameter, number of leaves, leaf area, stover dry weight, and root dry weight of soybean plants up to 35 days after planting and increase B. subtilis population grew from 1010 CFU.g-1 to be 4.43x1011 CFU.g-1. The presence of Trichoderma supports the activity of B. subtilis in the rhizosphere of the Detam variety soybean plantation.