E3S Web Conf.
Volume 233, 20212020 2nd International Academic Exchange Conference on Science and Technology Innovation (IAECST 2020)
|Number of page(s)||4|
|Section||BFS2020-Biotechnology and Food Science|
|Published online||27 January 2021|
Effect of high-voltage electrostatic field on cryopreservation of human epidermal melanocytes
1 Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing, China
2 College of Food Science and Engineering, Jilin Agricultural University, Changchun, China
a Correspondence author: Jintian Tang, E-mail, firstname.lastname@example.org
The objective of this research was to evaluate the influence of high-voltage electrostatic field (HVEF) on the freezing of human epidermal melanocytes (HEM) with respect to the degree of cell deformation, survival and proliferation rate after cell resuscitation. As a result, the degree of supercooling is increased by enhancing the strength of the static electric field in the range of 15 kV/m, and the maximum supercooling degree is 7.83±0.05 °C at 15 kV/m. By contrast, the morphology of the electric field assisted freezing cell after resuscitation was significantly improved, and the best electric field strength for cryopreservation is 15 kV/m. The survival rate of human epidermal melanocytes recovered was 88.03%, which was higher than that of the control group. The proliferation rate at 24, 48 and 72 hours are 17%, 28% and 25%, respectively, which are higher than that of the control cells. These findings demonstrate that the freezing HVEF can protect the cell physiological activity, and reduce the freezing damage. Therefore, the optimal HVEF cryopreservation technology be of great significance for the research of tissue engineering in repairing wounds, infections, and promote the development of food, medicine and other fields.
© The Authors, published by EDP Sciences 2021
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