E3S Web Conf.
Volume 328, 2021International Conference on Science and Technology (ICST 2021)
|Number of page(s)||6|
|Section||Chemical Engineering, Physical Characteristics, Water Treatment|
|Published online||06 December 2021|
Total Phenol Content and Activities of Antioxidant Extracts Methanol Limes (Citrus Aurentifolia) By Uv-Vis Spectrophotometry
1 Chemistry Education Study Program, FKIP Khairun University, Ternate, Indonesia
2 Department of Chemistry, FMIPA, Hasanuddin University, Makassar, Indonesia
3 Department of Obstetrics and Gynecology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
4 Student from Department of Chemistry, FMIPA, Hasanuddin University, Makassar, Indonesia
* Corresponding author : email@example.com
Total phenol levels and antioxidant activity in whole limes from Palopo, Luwu Regency, as well as the relationship between total phenol content and antioxidant activity, were determined. Maceration of dried whole lime fruit yielded the methanol extract (Citrus aurentifolia). Phytochemical screening was used to determine the presence of alkaloids, phenolic compounds, and flavonoids in the extract’s secondary metabolites. The total phenol extract was determined using the Folin-Ciocalteu method, yielding a value of 8.37 mgGAE/g. Antioxidant activity was determined using the DPPH method, and the IC50 value was 1793.06 g/mL. Total phenol content of whole fruit extract lime and IC50 show a linear relationship y = -168,11x + 3211 with a determination R2 = of 0.9780.
Key words: Uv-Vis Spectrophotometry / Antioxidant Activity / Phytochemical screening
© The Authors, published by EDP Sciences, 2021
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