E3S Web Conf.
Volume 249, 20214th International Conference on Sustainability Science (CSS2020)
|Number of page(s)||5|
|Section||Conservation, Resilience, Environmental Vulnerability and Hazard|
|Published online||07 April 2021|
Phylogenetic of Four Bamboo Genera Based on NdhH Sequences, Chloroplast DNA
Department Biology, Faculty of Mathematics and Natural Sciences Natural Science, Universitas Padjadjaran, Indonesia
* Corresponding author : firstname.lastname@example.org
Bamboos are versatile plants with many usages. Indonesia has a high diversity of Bamboos species and has been using them for a long time. As multipurpose plants, they are vulnerable to over exploration. Much of the bamboo land has been converted into housing or agricultural land. Development and conservation need to be balanced, to protect the genetic diversity, bamboo in particular. We studied the relationship among four genera of bamboo that mostly found in Indonesia using gene that presents in chloroplast DNA, ndhH. DNA from twenty-four samples from Bambusa, Dendrocalamus, Gigantochloa and Schizostachyum were extracted and amplified using specific ndhH primer. Sequencing results were analysed with BioEdit 7.2 software. Phylogenetic tree was build using raxmlGUI 2.0 software. The result showed that ndhH gene generated good sequence quality. It has small variation among samples. The result suggested that ndhH was able to differentiate some species within Schizostachyum, however; it was not a success for others.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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