DNA sexing for gender determination of Changeable Hawk-Eagle (Nisaetus cirrhatus, Gmelin, 1788)

¹ Department Biology, Faculty of Mathematics and Natural Sciences Natural Science, Universitas Padjadjaran, Indonesia
² Center for Environment and Sustainability Science, Universitas Padjadjaran, Indonesia
³ Environment Science and Sustainability Science Program, Postgraduate School, Universitas Padjadjaran, Indonesia

^* Corresponding author : annisa18040@mail.unpad.ac.id

Abstract

The Changeable hawk-eagle (Brontok eagle) is a protected bird species. It is one of the most frequently hunted and traded birds in Indonesia. The processes of being traded changes this bird natural behaviour. Therefore, a rehabilitation effort to return the eagle’s behaviour to conform to its natural habits is needed. The ultimate goal of rehabilitation is to release the changeable hawk-eagle back into its natural habitat. In conservation and breeding programs, efforts to determine the sex of eagles to be released are very important to help increase the population of changeable hawk-eagles in their habitat by looking at the sex ratio. At the present, sex determination at the Kamojang Conservation Eagle Center (Pusat Konservasi Elang Kamojang or PKEK) uses the morphometric method. This research used the DNA sexing method with primers 2550F and 2718R to determine the sex of Changeable hawk-eagles in PKEK by extracting DNA from blood samples of 30 eagles. Comparison of DNA sexing results and morphometric data showed differences. This proves that DNA sexing, is suitable in determining changeable hawk-eagles’ sex.