E3S Web Conf.
Volume 252, 20212021 International Conference on Power Grid System and Green Energy (PGSGE 2021)
|Number of page(s)||5|
|Section||Research and Development of Electrical Equipment and Energy Nuclear Power Devices|
|Published online||23 April 2021|
Quick extraction and direct determination of amino acids from plants by hydrophilic interaction liquid chromatography (HILIC) and high-performance liquid chromatography-mass spectrometry without derivatization
1 School of Chemical Engineering, Sichuan University, Chengdu 610065, China
2 National Institute of Measurement and Testing Technology, Chengdu 610021, China
Amino acids are naturally compound in many plants and have a essential effects on human life. This work presents a simple and rapid analytical method for direct determination of amino acids without derivatization. The preparation method of samples was green and quick, which extracted amino acids with water in 10 min, and separated on a HILIC-Z (2.1 mm×100 mm, 2.7 μm i.d.) using acetonitrile-water solution as mobile phase, and then detected under ESI-multiple reaction monitoring mode. Satisfactory determination of 21 underivatized amino acids from three different plants was achieved in 15 min. This method showed a good linearity (R2 > 0.99) for the analyst and the detection limits (LOD) were 0.53 mg/kg–10.39 mg/kg for the amino acids. The average recoveries were in the range of 80%–120% at spiked concentrations. The analytical method is high sensitivity, accuracy and analytical efficiency, meanwhile it may be used for the analysis of amino acids in other plants.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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