| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
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|---|---|---|
| Article Number | 01012 | |
| Number of page(s) | 13 | |
| Section | Genetic Resources Are the Basis of Selection and Seed Production in the Development of Organic Agriculture | |
| DOI | https://doi.org/10.1051/e3sconf/202125401012 | |
| Published online | 05 May 2021 | |
Research of 5 extracts of wild Amur grape Vitis amurensis Rupr. and identification of its polyphenolic composition by tandem mass spectrometry (HPLC-MS / MS)
1 N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya, 42–44, 190000 Saint-Petersburg, Russian Federation
2 Far Eastern Federal University, Sukhanova, 8, 690950 Vladivostok, Russian Federation
3 Pacific Geographical Institute, Far Eastern Branch of the Russian Academy of Sciences, Radio, 7, 690041 Vladivostok, Russian Federation
4 Siberian Federal Scientific Centre of Agrobiotechnology, Centralnaya, Presidium, 633501 Krasnoobsk
* Correspondence: razgonova.mp@dvfu.ru
Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for the first time, a comparative metabolomic study of biologically active substances of wild grapes collected from five different places of the Primorsky and Khabarovsk territories is carried out. To identify target analytes in ethanol extracts of grape berries, high performance liquid chromatography (HPLC) was used in combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped with an ESI electrospray ionization source in negative and positive ion modes. The mass spectrometer was used in the scan range m / z 100 - 1.700 for MS and MS / MS. Used fragmentation of the 4th order. Primary mass spectrometric results showed the presence of 89 biologically active compounds corresponding to the species V. amurensis, moreover, salvianolic acids F, D and G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan identified for the first time in V. amurensis.
© The Authors, published by EDP Sciences, 2021
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