Phenotypic and genotypic features of strains of Actinobaculum suis- the causative agent of urinary tract infections in pigs

Federal State Budgetary Educational Institution of Higher Education “Omsk State Agrarian University named after P.A. Stolypin”, Omsk, Russian Federation

^* Corresponding author: vi.pleshakova@omgau.org

Abstract

urinary tract infections (UTI) are a significant problem of infectious pathology among the pig population. Many researchers consider UTI in pigs as a result of the influence of conditionally pathogenic microorganisms and unfavorable conditions of keeping and feeding. In the middle of the last century, a number of researchers identified a specific pathogen, namely Actinobaculum suis, which causes a pathological condition of the bladder and kidneys in pigs. The microorganism remains poorly studied. In morphometric and biochemical terms, it has a weak variance of phenotypic traits, with the greatest fluctuations in the enzyme profiles of the isolated cultures. It is shown that the size of microbial bodies has a direct dependence not only on the component composition of the growth medium, but also on the native pathological material from which the microorganism was isolated. Molecular genetic studies have shown that the content of GC-pairs was on average 55.5 mole%, which corresponds to the taxonomic position of the pathogen. At the same time, 16SpRNA restriction analysis did not reveal differences in PCR products, which in turn indicates a high degree of genetic relation of the isolated Actinobaculum suis cultures.