E3S Web Conf.
Volume 267, 20217th International Conference on Energy Science and Chemical Engineering (ICESCE 2021)
|Number of page(s)||4|
|Section||Environmental Chemistry Research and Chemical Preparation Process|
|Published online||04 June 2021|
Recombinant protein expression and purification experiments based on SARS-CoV-2 Mpro
Academy of our lady of mercy-Lauralton Hall
corresponding author’s email address: firstname.lastname@example.org
Mpro is one of the most important drug targets of the novel Coronavirus. In this study, a series of potential compounds were obtained through virtual drug screening targeting Mpro. Using the key protease (Mpro) of SARS-COV-2 as the drug lead target for virtual drug screening has become a hot topic in current virus research. Mpro plays a key role in virus replication and transcription. Therefore, the key experimental strain of Escherichia coli containing Mpro is commonly used to inhibit drugs from Mpro. In this study, Mpro gene was located on peT-28A vector, and the size of the recombinant protein was about 36 kDa. The e. Coli expression strain BL21(DE3) containing Mpro was inoculated and cultured.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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