| Issue |
E3S Web Conf.
Volume 271, 2021
2021 2nd International Academic Conference on Energy Conservation, Environmental Protection and Energy Science (ICEPE 2021)
|
|
|---|---|---|
| Article Number | 03060 | |
| Number of page(s) | 4 | |
| Section | Research on Energy Chemistry and Chemical Simulation Performance | |
| DOI | https://doi.org/10.1051/e3sconf/202127103060 | |
| Published online | 15 June 2021 | |
Rapid antimicrobial susceptibility testing from positive blood cultures based on Stimulated Raman Scattering Imaging analysis
1 Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
2 Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
* Corresponding author: kangxx@vip.sina.com
The existing identification (ID) and antimicrobial susceptibility testing (AST) method requires at least two to three days to detect blood infection, and a fast and accurate detection method is very necessary for sepsis patients or Intensive Care Unit (ICU) patients. Here, we describe a direct isolated bacteria from a positive blood culture bottle (PBCB), and rapid AST method by femtosecond stimulated Raman scattering (SRS) imaging of deuterium oxide (D2O) metabolism, which can determine the antimicrobial susceptibility of bacteria from PBCB in 5-6 hours. The positive blood culture sample is passed through a filter membrane and mixed with cell lysis, after through the centrifugal which can directly isolated bacterium in order to identification by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), following by antibiotic susceptibility testing by SRS imaging within a day. Overall, this rapid and rapid process combination of MALDI-TOF MS and SRS imaging of deuterium oxide (D2O) metabolism can solve the direct identification and antibiotic susceptibility testing of pathogen in positive blood cultures.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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