| Issue |
E3S Web Conf.
Volume 285, 2021
International Conference on Advances in Agrobusiness and Biotechnology Research (ABR 2021)
|
|
|---|---|---|
| Article Number | 03013 | |
| Number of page(s) | 4 | |
| Section | Agricultural Biotechnology and Chemistry | |
| DOI | https://doi.org/10.1051/e3sconf/202128503013 | |
| Published online | 06 July 2021 | |
Effect of cAMP on bovine oocyte maturation in vitro
1
Ural Federal Agrarian Research Center of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russia
2
Center of Family Medicine, Yekaterinburg, Russia
* Corresponding author: isaeva.05@bk.ru
Cyclic nucleotides cAMP and cGMP are among the main molecules that control the maturation of mammal oocytes. The in vitro simulated physiological oocyte maturation (SPOM) method was used to model cAMP accumulation in the oocyte. In the current study, we preincubated the oocytes of cows (not primed with FSH) with cAMP modulators: N 6,2’-O-dibutyryladenosine 3’,5’-cyclomonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX). The use of SPOM increased the yield of bovine blastocysts.
© The Authors, published by EDP Sciences, 2021
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