E3S Web Conf.
Volume 290, 20212021 3rd International Conference on Geoscience and Environmental Chemistry (ICGEC 2021)
|Number of page(s)||4|
|Section||Environmental Chemistry and Chemical Technology Application|
|Published online||14 July 2021|
Determination of Isoniazid by Fe(II)-2,2’-Bipyridine Spectrophotometry
School of Chemistry and Environment, Jiaying University, Meizhou, Guangdong 514015, China Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meizhou, Guangdong 514015, China
* Corresponding author: email@example.com
In acidic media, Fe(Ш) could be reduced to Fe(Ⅱ) by isoniazid, Fe(Ⅱ) reacts with 2,2'-bipyridine to form an orange complex with a maximum absorption wavelength of 522 nm. According to this principle, the content of isoniazid can be measured indirectly by measuring the amount of Fe(Ⅱ). A new method for the determination of isoniazid by Fe(Ⅱ)-2,2'-bipyridine spectrophotometry has been established. At the optimum conditions, the linear relationship is good between the mass concentration of isoniazid and absorbance of Fe(Ⅱ)-2,2'-bipyridine complex in the range of 0.0004000-0.002800 mg/mL. The linear regression equation is A=0.0251+138.04C(mg/mL), and the linear correlation coefficient is 0.9995. This method is used to determine the content of isoniazid in isoniazid tablets, and the results are similar to those obtained by pharmacopoeia method.
© The Authors, published by EDP Sciences, 2021
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