| Issue |
E3S Web Conf.
Volume 292, 2021
2021 2nd International Conference on New Energy Technology and Industrial Development (NETID 2021)
|
|
|---|---|---|
| Article Number | 03087 | |
| Number of page(s) | 4 | |
| Section | Environmental Sustainable Development and Industrial Transformation | |
| DOI | https://doi.org/10.1051/e3sconf/202129203087 | |
| Published online | 09 September 2021 | |
Expression of human G3BP1 in E. coli
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China
* Corresponding author: ziweiyang@tju.edu.cn
G3BP1 (Ras-GTPase-activating protein SH3 domain-binding protein) is responsible for normal RNA stress granule (SG) assembly and overexpressed in many cancer cells. Deletion of G3BP1 decreases the number and size of SGs. SGs are complex of RNA and proteins that stall translation of protein in response to stress. Given the function of G3BP1 in stress granule assembly and tumor suppression, it is believed that G3BP1 regulates cell growth and proliferation as well. Here, I constructed the recombinant protein expression vector and systemically optimized condition for the expression of human G3BP1 protein in E. coli. This research should be useful for investigating further functional analysis and atomic structure of G3BP1.
© The Authors, published by EDP Sciences, 2021
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