| Issue |
E3S Web Conf.
Volume 293, 2021
2021 3rd Global Conference on Ecological Environment and Civil Engineering (GCEECE 2021)
|
|
|---|---|---|
| Article Number | 01028 | |
| Number of page(s) | 5 | |
| Section | Ecological Environment Protection and Pollution Control | |
| DOI | https://doi.org/10.1051/e3sconf/202129301028 | |
| Published online | 23 July 2021 | |
Isolation and identification of a high-efficiency phenol-degrading bacteria and optimization of its degradation conditions
1 College of Geography and Environmental Engineering, Lanzhou City University, Lanzhou Gansu 730070, China
2 School of Biological and Environmental Engineering, Xi’an University, Xi’an 710065, China
* Corresponding author: Haili Sun and Tianpeng Gao, Emil: lzusun@163.com(H. S.), zkgtp@163.com(T. G.)
Phenol is widely used in China, it not only pollutes the environment, but also accumulates toxic substances in the human body through the food chain, further harming humans. In this experiment, a strain of high-efficiency low-temperature degradation phenol bacteria B5 was selected from the soil contaminated by organic matter of Lanzhou. Through research methods such as Gram staining observation, DNA extraction, PCR amplification, sequencing and comparison, it was found that this strain was Pantoea agglomerans. Through the subsequent optimization of degradation conditions, it was found that the B5 strain can degrade 500mg/L of phenol to 24.8mg/L in 36h. The ability to degrade phenol is stronger between pH5.5-pH6.0, and the ability to degrade phenol is higher in a medium containing 4-8g/L sodium chloride. This research can provide certain theoretical guidance for phenol degradation.
© The Authors, published by EDP Sciences, 2021
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