E3S Web Conf.
Volume 306, 2021The First International Conference on Assessment and Development of Agricultural Innovation (1st ICADAI 2021)
|Number of page(s)||9|
|Published online||24 September 2021|
Cytological diversity in colchiploid of Pontianak tangerine citrus
Indonesian Citrus and Subtropical Fruits Research Institute, Jl. Raya Tlekung no 1, Junrejo, Batu, East Java, Indonesia
* Corresponding author: email@example.com
Pontianak tangerine citrus (Siam Pontianak) is the most popular tangerine citrus in Indonesia. Induced mutation with colchicine treatment on Pontianak tangerine was intended to produce polyploid plants. The study aimed to analyze the cytological diversity in colchiploid of Pontianak tangerine citrus. The study was conducted at ICISFRI from January – May 2018. The cytological analysis was performed on a five-year-old of 36 colchiploids of Pontianak tangerine and a mother plant as control. The results showed that the colchiploid plants had higher chromosome numbers, stomatal sizes, and chloroplast numbers than the control. However, the stomatal density in several genotypes did not differ from that of the control. Colchicine treatment resulted in an aneuploid plant with chromosome number 18+x. Based on the clustering analysis results, all colchiploid plants differed from the control with a variability coefficient of 0.65. The colchiploid plants were not grouped according to colchicine concentration and duration of treatment. The increase of colchicine concentration and treatment duration did not correlate with the increasing ploidy number of colchiploid plants. The implication of the research result was the stomatal density, stomatal size, and chloroplast number were useful tools for rapid pre-screening of plant polyploidy.
© The Authors, published by EDP Sciences, 2021
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