| Issue |
E3S Web Conf.
Volume 319, 2021
International Congress on Health Vigilance (VIGISAN 2021)
|
|
|---|---|---|
| Article Number | 02012 | |
| Number of page(s) | 8 | |
| Section | Methods, Tools and Techniques in Health Vigilance | |
| DOI | https://doi.org/10.1051/e3sconf/202131902012 | |
| Published online | 24 November 2021 | |
Systematic review and meta-analysis for the biotechnological production of THC in Morocco
1 Research Laboratory Innovation & Information System, EuRSED Vienne France.
2 UIT, University Ibn Tofaıl, Kenitra Morocco
3 PHI, Pharmaceutical Institut, Ain El Aouda Morocco
4 UIT, University Ibn Tofaıl, Kenitra Morocco
5 CNRST, Center for Scientific and Technical Research, Rabat Morocco
6 IAGGR, Institute of Genetic Analysis in the Royal Gendarmerie, Rabat Morocco
* Corresponding author: fadwa.badrana@uit.ac.ma
Cannabinoids have promising therapeutic potential. Δ9-tetrahydrocannabinol (THC), the most important psychotropic active ingredient of Cannabis Sativa L, has been the subject of several chemical, pharmacological and biosynthetic studies. In this context, a meta-analysis of biotechnological processes applied to the production of recombinant cannabinoid THC worldwide was carried out. The objective was to highlight the potential of these processes on the Moroccan variety of Cannabis Sativa. The PubMed, ScienceDirect and Web of Science search motors were used to search for original scientific work presenting biotechnological tools used in the production of THC. The scientific articles exploited are those published before the end of 2020. Succinct analysis of the experimental work performed showed that expression of the gene encoding Cannabis Sativa L. THCA synthase was performed on prokaryotic and eukaryotic expression systems. Currently, no functional expression could be obtained in E. coli. Whereas, production of recombinant protein (THCA Synthase) associated with significant enzymatic activity was obtained in P. pastoris cultures (F. Saccharomycetaceae). The exploitation of the sequences showed the presence of a large similarity between the THCA Synthase gene of cannabis Sativa of the Moroccan variety and the mRNA precursor of the same gene reported in several studies. This will allow us to use a specific signal sequence of choice for an adopted expression host, in order to produce the recombinant THCA synthase enzyme from the Moroccan Cannabis Sativa L strain on P. pastoris cultures.
© The Authors, published by EDP Sciences, 2021
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