Issue |
E3S Web Conf.
Volume 371, 2023
International Scientific Conference “Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East” (AFE-2022)
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Article Number | 01032 | |
Number of page(s) | 7 | |
Section | Smart Farming and Precision Agriculture | |
DOI | https://doi.org/10.1051/e3sconf/202337101032 | |
Published online | 28 February 2023 |
Invitro rearing of parasitoids
Tashkent State Agrarian University, 100140 Tashkent, Uzbekistan
* Corresponding author: jumaevrasoul@yandex.ru
Since 2015, the studies on in vitro rearing Braconidae and Trichogrammatidae (B. habetor Say, B. greeni and T. pintoe, T. evanescens, T. chilonis) have been conducted successfully in Uzbekistan. For these parasites, cooking and the three main types of media were made from the hemolymph of the wax moth (G. melonnella) and cotton worm Helicoverpa armigera (Heliothis armigera), egg yolk, natural milk 10%. The simulated "host-eggs" and "larvae" are made of polyethylene or polypropylene semi spherical capsules, containing artificial diets, in which insectan pupal hemolymph is the main component. Mass production of in vitro reared Braconidae and Trichogrammatidae its utilization in the fields showed good effectiveness in controlling cotton bollworm. In the article researches were given about creating artificial medium of diets in order to grow bracon and choosing effective medium of diets. In our researches medium of diets which consists of some components was created and they were harmed with bracon generations. According to it, generation of bracon’s nutrition and developing three main types of medium were produced in order to rear bracon parasite that was composed wax moths (G.melonnellan) and (heliothis armigera) hemolymph, egg yolk, natural milk.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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