| Issue |
E3S Web Conf.
Volume 371, 2023
International Scientific Conference “Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East” (AFE-2022)
|
|
|---|---|---|
| Article Number | 01090 | |
| Number of page(s) | 5 | |
| Section | Smart Farming and Precision Agriculture | |
| DOI | https://doi.org/10.1051/e3sconf/202337101090 | |
| Published online | 28 February 2023 | |
Induction of direct organogenesis in Blackberry (Rubus fruticosus L.) using leaf explants
RUDN University, 117198 Moscow, Russia
* Corresponding author: mfreddy.28fm@gmail.com
This study is to establish the best conditions for in vitro propagation by inducing direct organogenesis of “Thornfree” blackberry (Rubus fruticosus L.) using different doses of plant growth regulators, which affect the regeneration of shoots in compound nutrient medium of mineral salts Murashige and Skoog (MS). Young leaves were used as explants. In the initiation phase, plant material necessary for the experiment was obtained, using an MS nutrient medium enriched with 0.5 mg. l-1 of BAP, after 30 days’ leaves were obtained that were introduced in regeneration medium where the effect of BAP (1 and 2 mg. l-1) was evaluated in combination with AIA (0.5 mg. l-1) and TDZ (0.5 mg. l-1). The highest number of shoots with an average of 6.40 and oxidation percentage of 7.41% was achieved. In the proliferation phase no significant statistical differences were observed, however, the optimum performance attained for the number of buds was obtained with a concentration of 2 mg. l-1 of BAP, having an average shoot-length of 6.10 cm and a rooting percentage of over 80%.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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