| Issue |
E3S Web of Conf.
Volume 373, 2023
ISEPROLOCAL 2022 - The 3rd International Seminar on Promoting Local Resources for Sustainable Agriculture and Development
|
|
|---|---|---|
| Article Number | 03029 | |
| Number of page(s) | 5 | |
| Section | Crop Production and Breeding | |
| DOI | https://doi.org/10.1051/e3sconf/202337303029 | |
| Published online | 14 March 2023 | |
Karyotype of Phaius tankervilleae and Phaius amboinensis orchid
1 Sebelas Maret University, Department of Agrotechnology, Faculty of Agriculture, Surakarta, Indonesia
2 Sebelas Maret University, Plant Breeding Laboratory, Agrotechnology Study Program, Faculty of Agriculture, Surakarta, Indonesia
3 Sebelas Maret University, Center for Research and Development of Biotechnology and Biodiversity (P3BB), Institute for Research and Community Service (LPPM), Surakarta, Indonesia
* Corresponding author: tatik_oc@yahoo.com
Phaius tankervilleae and Phaius amboinensis orchids are decorative orchids that have high ornamental value and are classified as endangered plants. Genetic information from the P. tankervilleae and P. amboinensis orchids will be useful for plant breeders to develop a strategy in the ornamental improvement program. The aim of the study was to determine the karyotype pattern of P. tankervilleae and P. amboinensis orchids. Chromosomes obtained from meristematic root tips of each species were used to construct a karyotype and idiogram used the squash method. The results showed that the chromosome numbers and karyotype formulae of P. tankervilleae was 2n=2x=44=44 m (metacentric) and P. amboinensis was 2n=2x= 46=44m+2sm (sub-metacentric). Intrachromosomal Asymmetry Index (A1) of P. tankervillae was 0,10 and P. amboinensis was 0,15, indicates that both of them tend to have a metacentric chromosome type. Interchromosomal Asymmetry Index (A2) of P. tankervillae was 0,37 and P. amboinensis was 0,34, indicates that both have sufficient varies in size of chromosomes.
© The Authors, published by EDP Sciences, 2023
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