| Issue |
E3S Web Conf.
Volume 466, 2023
2023 8th International Conference on Advances in Energy and Environment Research & Clean Energy and Energy Storage Technology Forum (ICAEER & CEEST 2023)
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|
|---|---|---|
| Article Number | 01013 | |
| Number of page(s) | 5 | |
| Section | Energy Material Research and Power Generation System Analysis | |
| DOI | https://doi.org/10.1051/e3sconf/202346601013 | |
| Published online | 15 December 2023 | |
Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge
School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei China
* Corresponding author: ningzhifang@163.com
Excess sludge a resource-rich organic waste, can be solubilized by thermophilic enzymes to extract proteins and anaerobic digestion of methane production to reduce sludge and use resources. At the same time, it can improve new ideas to relieve the pressure of the energy industry. To solve the problems of low enzyme-producing activity of wild strains and low hydrolysis rate of ES, this study utilized UV-DES compound mutagenesis to screen dominant mutant strains under optimized conditions, which were added to ES hydrolysis reaction system. The results showed that UV-DES mutagenesis could effectively improve the ability of S-TE and promote the hydrolysis of ES. The protease activity of the mutant strain KT16 obtained by screening was 46.7% higher than that of the original strain DC8. KT16 effectively degraded insoluble organic matter, reduced the average particle size of ES, and increased the concentrations of extracellular proteins, polypeptides, and amino acids to 1731.8, 521.3, and 510 mg·L-1, respectively. This study will provide a reference for the resource utilized.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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