Antioxidant Activity Between Defatted and Different Solvent Temperature in Rice Bran var. IR-64 Extract

¹ Agricultural Product Technology, University Veteran Bangun Nusantara, Jl. Letjend. S. Humardani No.1, Sukoharjo 57521, Central Java, Indonesia
² Agricultural Product Technology, University Veteran Bangun Nusantara, Jl. Letjend. S. Humardani No.1, Sukoharjo 57521, Central Java, Indonesia
³ Department of Biotechnology, Quaid-i-Azam University, NCB Building Islamabad 45320, Pakistan
⁴ Pakistan Academy of Sciences, 3 Constitution Ave, G-5/2, Islamabad Capital Territory, Pakistan

^* Corresponding author: srihartati@univetbantara.ac.id

Abstract

The extraction of bioactive components in rice bran is influenced by various factors, including defatted treatment and temperature. The aim of this study was to know the yields, total phenol content, and antioxidant activity by defatted and solvent temperature treatment extraction in rice bran. The defatted treatment was not significant effect on the antioxidant activity of rice bran extract. Temperature treatment significantly increased antioxidant activity in rice bran extract. The yield obtained in the control (non-defatted) was 17.19 % with antioxidant activity of 66.30 % and phenol content of 148.6 mg GAE g⁻¹ extract. While the yield on the defatted treatment showed a result of 0.49 % with antioxidant activity of 67.33 % and a phenol level of 53.34 mg GAE g⁻¹ extract. The room temperature treatments on 50 °C and 70 °C obtained yields around 17.19 %; 9.81 % and 7.34 % with antioxidant activity 67.3 %; 82.09 % and 77.83 % and phenol content was 148.6 mg GAE g ⁻¹ extract, 91.7 mg GAE g ⁻¹ extract and 59.8 mg GAE g ⁻¹ extract. Defatted treatment was not significant effect on the antioxidant activity. Therefore, the optimum temperature of solvent that was effective to the antioxidant activity of rice bran IR-64 extract was at 50 °C.