Study of the Oxolin antiviral drug effect for the improvement of potato seed material during in vitro cultivation

State Agricultural Academy of Velikie Luki, Velikie Luki, Russia

¹ Corresponding author: nich@vgsa.ru

Abstract

The article presents an analysis of the potato breeding problems in Russia which are associated with the low phytosanitary state of potato seed material infected with viral and bacterial diseases, as a result, it is necessary to carry out its improvement and the use of Russian varieties. Experiments were conducted in the laboratory of plant microclonal propagation according to the methodological recommendations for improvement and accelerated propagation of seed potatoes. The results of research on refining the technology of seed potato improvement by adding the Oxolin antiviral drug to the standard nutrient medium MS; its optimal concentrations for obtaining the largest number of uninfected plants of the studied varieties were identified. For potato varieties Liga, Hussar, and Real, the optimal concentration was 20 mg/l of Oxolin, while in the varieties Aurora, Real, and Sirenevy tuman, the concentration was 30 mg/l. Therefore, it is necessary to search for antiviral drugs with an alternative mechanism of action which remains very relevant and practically significant. The research results obtained in this work can be applied both to optimize the cultivation regime of potato microclones in vitro and to enhance the productive process of the uninfected microplants.