Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam

Department of Biotechnology, Joint Vietnam Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen, Nghia Do, Cau Giay, Ha Noi, Vietnam

^* Corresponding author: tuyendodhkh@gmail.com

Abstract

The study was carried out to isolate and select useful thermophilic yeast strains in the process of organic domestic waste treatment in Phu Luong - Thai Nguyen. Research results from 23 samples of rust have isolated 10 strains of yeast on YPG medium at 40 oC. Among them, 6 strains of yeast were selected with the ability to grow and develop in a wide temperature range from 20-45 oC. The results of identification combined with morphological, physiological, and biochemical characteristics of yeast strains showed that, out of 6 selected strains, there were 3 strains belonging to the genus Saccharomyces (Saccharomyces sp. TNY13.01, Saccharomyces sp. TNY22.01), Saccharomyces cerevisiae TNY13.09), 2 strains of the genus Candida (Candida sp. TNY23.01, Candida tropicalis TNY23.126) and 1 strain of the genus Papiliotrema (Papiliotrema laurentii TNY23.127). Among them, the identified strain Saccharomyces cerevisiae TNY13.09 has the ability to grow at 45, tolerates a wide pH range of 4.0– 8.5, has a positive catalase reaction, is capable of using a variety of carbon sources, and belongs to class I biosafety group. On that basis, Saccharomyces cerevisiae TNY13.09 has the potential to be further researched and applied as additional microbial inoculants to the organic waste heap.