In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

¹ Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Russia
² Federal State Funded Institution of Science, The Labor Red Banner Order Nikita Botanical Gardens – National Scientific Center of the RAS, 298648 Yalta, Russia

^* Corresponding author: bilyak_alla@mail.ru

Abstract

The article discusses the introduction in vitro, selection of the nutrient medium and plant growth regulator effect on the microshoot development of the perspective fruit crop Prunus persica ‘Dostoyniy’. Murashige and Skoog (MS), Gamborg and Eveleg (B5), Blaydes (BL), McCown (WPM) media supplemented with 0.5 mg/l 6-BAP, 0.1 mg/l IBA; 1.0 mg/l 6-BAP, 0.025 mg/l IBA; 0.48 mg/l KIN, 0.1 mg/l IBA; 0.96 mg/l KIN, 0.025 mg/l IBA and succinic acid were used in our research. According to obtained results, the most optimal season for the introduction of P. persica ‘Dostoiniy’ into in vitro culture was summer. The use of alcohol-, and chlorine-containing sterilizing agents in combination with antibiotics (or without them) provides aseptic material. BL and B5 media supplemented with 0.5 mg/l 6-BAP and 0.1 mg/l IBA, as well as 1.0 mg/l 6-BAP and 0.025 mg/l IBA promote microshoot development in vitro. Succinic acid in the culture medium at concentration of 5 and 10 g/l contributes to an additional increase in the multiplication rate.