Issue |
E3S Web of Conf.
Volume 462, 2023
International Scientific Conference “Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East” (AFE-2023)
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Article Number | 01018 | |
Number of page(s) | 7 | |
Section | Advances in Precision Livestock Farming | |
DOI | https://doi.org/10.1051/e3sconf/202346201018 | |
Published online | 12 December 2023 |
Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle
1 Tatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of Sciences, 48, Orenburg tract st., 420059 Kazan, Russian Federation
2 N.E. Bauman Kazan State Academy of Veterinary Medicine Federal State Budgetary Institution of Higher Education, 35, Siberian tract st., 420029 Kazan, Russian Federation
* Corresponding author: natysafina@gmail.com
The study examined the polymorphism of the glutathione peroxidase-1 gene (GPX-1, g. 189 T/C) effect on the GPx enzyme activity and biochemical parameters in the blood serum of Holstein cows of indigenous and foreign selection. The work was carried out in the Lenin Breeding Farm Integrated Agricultural Production Center (IAPC) and the Mukhametshin Z.Z. Peasant Farm Enterprise (PFE) of the Republic of Tatarstan. DNA genotyping by the locus of the Bos taurus GPX-1-Bsc4 I gene was performed by PCR-RFLP. As a result of genetic typing of cattle, 2 alleles (C and T) and 3 genotypes (CC, TC and TT) were identified. The classification carried out by PIC values in two populations of Holstein cattle showed that there is a moderate polymorphism of the GPX-1 gene. A negative inbreeding coefficient (F) indicates excessive outbreeding in both populations. In both populations, the numerical advantage of heterozygous genotype TC cows is 60.1 and 56.7 % of the total livestock. Indigenous breeding cows, compared with imported cattle had higher: GPx activity, AST, ALT, cholesterol level, total protein and albumin fraction. Analysis of the association between the GPX-1 gene polymorphism and the GPx enzyme level indicates that the activity of this enzyme varies depending on the genotype, and there is also a variation in the biochemical parameters of the Holstein cattle blood serum.
© The Authors, published by EDP Sciences, 2023
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