| Issue |
E3S Web of Conf.
Volume 462, 2023
International Scientific Conference “Fundamental and Applied Scientific Research in the Development of Agriculture in the Far East” (AFE-2023)
|
|
|---|---|---|
| Article Number | 01021 | |
| Number of page(s) | 12 | |
| Section | Advances in Precision Livestock Farming | |
| DOI | https://doi.org/10.1051/e3sconf/202346201021 | |
| Published online | 12 December 2023 | |
Exploring the potential of Bacillus subtilis as an additive for decontamination of feed
1 All-Russian Research Institute of Phytopathology SSI, Russian Agricultural Academy, Department of Ecology, 143050 Bolshie Vyazemy, Odintsovo distr., Moscow reg., Russia
2 Federal Center for Toxicological, Radiation and Biological Safety, Department of Biotechnology, 420075 Kazan, Russia
* Corresponding author: valiullin27@mail.ru
Fusariotoxins (deoxynivalenol, nivalenol, T-2 toxin, diacetoxyscirpenol, zearalenone, moniliformin, fumonisins) are secondary metabolites of Fusarium species of fungi that parasitize various plants or are present on them as facultative pathogens. The aim of this work was to evaluate the ability of bacteria that have antimicrobial activity against toxin-producing Fusarium species to detoxify toxic fungal metabolites in order to create additives that improve the quality and safety of feed and other agricultural products. A total of 207 bacterial isolates belonging to various genera and species have been studied. The isolate, designated as RF-45, was characterized by a broad spectrum and a high level of antagonistic activity against most species of Fusarium fungi. Based on morphological-physiological and biochemical studies, analysis of the 16S rRNA gene sequence, the RF-45 isolate was identified as Bacillus subtilis. The bacillary isolate was distinguished by its ability to produce various hydrolytic enzymes (CMCase, avicelasa, β-glucosidase, FPase and xylanase) and antimicrobial metabolites (siderophores, bacteriocins and exopolysaccharides), as well as to detoxify toxic fungal metabolites. It was concluded that B. subtilis RF-45 can be used as an object for creating additives capable of decontaminating feed and other agricultural products.
Key words: Bacillus subtilis / fusariotoxins / additive / decontamination / feed / agricultural products
© The Authors, published by EDP Sciences, 2023
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