Issue |
E3S Web Conf.
Volume 477, 2024
International Conference on Smart Technologies and Applied Research (STAR'2023)
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Article Number | 00073 | |
Number of page(s) | 7 | |
DOI | https://doi.org/10.1051/e3sconf/202447700073 | |
Published online | 16 January 2024 |
Comparative Analysis of 2-Methylimidazole and 4-Methylimidazole Exposure Effects on Zebrafish Larvae Mortality Rate
Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India. Pincode: 602105.
* Corresponding author: prakashkalpana067@gmail.com
The study aimed to compare zebrafish embryo mortality rates under different 2-Methylimidazole (2-MI) and 4-Methylimidazole (4-MI) concentrations. Two exposure groups were created at a 40μM concentration using egg water. Thirty individuals were divided into two groups: Group 1 received an environmentally relevant dose of 2-MI, while Group 2 was exposed to 4-MI. Each group’s embryos (n=15 per plate) were observed from 4 to 96 hours post fertilization (hpf), adhering to OECD guidelines. Statistical analysis using SPSS maintained a 95% confidence level, 0.01 ratio, 80% power, and 1:1 enrollment ratio. Results showed a marked increase in mortality rate (71.87±1.25) in ecologically exposed embryos compared to 2-MI (45.73±0.79), with a significant P-value of 0.000 (P<0.05). Additionally, zebrafish exposed to 4-MI displayed a 20% lower mortality rate than 2-MI, supported by a significance threshold of p = 0.000 (p<0.05). These findings highlight the detrimental impact of early exposure to 2-MI and 4-MI on zebrafish embryo mortality rates, signifying developmental and embryotoxic implications.
Key words: Innovative model Zebrafish / 2-Methylimidazole / 4- Methylimidazole / Mortality Rate / Toxicity / Environment
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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