Crude Oil Polluted Soil Bioremediation through Microbe Activity Utilization

Environmental Engineering Study Program, Faculty of Landscape Architecture and Environmental Technology, Universitas Trisakti, Jakarta, Indonesia

^* Corresponding author: astririnanti@trisakti.ac.id

Abstract

Environmental pollution by crude oil has become a serious problem all over the world with high level of oil spillage or leaks comes from damaged crude oil piping, tankers storage, offshore drilling, and illegal oil waste dumping cases. The objective of this research is to study crude oil polluted land recovery process with biotechnological approach. Technology to safely remove oil pollutant in the environment is bioremediation due to its low cost, high efficiency level, environmentally friendly, and sustainability. Three isolate bacteria namely Pseudomonas sp., Pseudomonas xanthomarina, and Arthrobacter nitroguajacolicus were utilized as bioremediation agents to perform land remediation with biostimulation-bioaugmentation (BS-BA) approach. After 25 days, 31,000 mg/kg of Total Petroleum Hydrocarbon (TPH) was decreased into 90-10000 mg/kg or equal to 67.7% to 99.70%. We also detected 14,000 mg/kg decrease of Polycyclic Aromatic Hydrocarbon (PAH) into 8 to 40 mg/kg with detection limit of 99.94% to % in just 5-10 days. TPH removal kinetic calculation by using Pseudomonas xanthomarina bacteria resulted YT, Kd, Yobs, and Ks respectively at 0.002/hour, 0.001/hour, 0.0361/hour, and 0.0002/hour, by using order 2 formula with regression value of 0.9482. We recommend conducting land farming processing which consists of 2 beds with volumes of 55 m3/bed in order to remediate 159 ton of crude oil polluted soil by utilizing 9.6 L of hydrocarbonoclastic bacteria for 3723 hours. Based on the findings, we concluded that bioremediation is available on crude oil polluted soil.