Issue |
E3S Web of Conf.
Volume 539, 2024
III International Conference on Agriculture, Earth Remote Sensing and Environment (RSE-III-2024)
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Article Number | 02017 | |
Number of page(s) | 7 | |
Section | Issues of Sustainable Development of Agriculture | |
DOI | https://doi.org/10.1051/e3sconf/202453902017 | |
Published online | 17 June 2024 |
Isolation and identification of Bacillus subtilis and its potential in biocontrol of phytopathogenic fungi
1 Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan
2 Tashkent State Pedagogical University named after Nizami, Tashkent, Uzbekistan
* Corresponding author: narmuhamedovamohira@gmail.com
In the work, based on morphological, cultural, biochemical properties and on the basis of the 16S rRNA gene, a local strain of bacteria Bacillus subtilis TM was isolated from saline soils of the Fergana region of Uzbekistan. The strain was registered with NCBI under the number: OR944512.1. It was revealed that the strain Bacillus subtilis TM exhibits high antagonistic activity against the phytopathogenic fungi Fusarium verticillioides, Fusarium solani, Aspergillus orchaceus, Alternaria alternata, Fusarium culmorium, Alternaria tenuissima with an inhibition zone of 40-60 mm. At the same time, the B. subtilis TM strain showed moderate antagonistic activity against Fusarium oxysporium, Penicillium chrysogenium (with an inhibition zone of 22-23 mm) and relatively less antagonistic activity against Cladosporium sp (with an inhibition zone of 12 mm).
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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