Search for motifs, associated with response to abscisic acid in promoters of genes of Quercus robur and Populus trichocarpa plants

Federal Scientific Center of Agroecology, Complex Melioration and Protective Afforestation of the Russian Academy of Sciences, Laboratory of Genomic and Postgenomic technologies, Volgograd, Russian Federation

^* Corresponding author: malov-v@vfanc.ru

Abstract

Climate changing and increasing anthropogenic impact put more and more fertile lands under treat of degradation. To counteract this process, diverse methods, including agroforestry, should be applied, which, regarding changing environmental condition, requires development of new drought resistant tree forms and varieties. Quercus robur and Populus trichocarpa are actively used for this cause on the territories of southern Russia, their genomes are sequenced and annotated automatically, which facilitates search for potential genes of interest, which impact drought tolerance. Abscisic acid is a key participant of water deficiency and other abiotic stress response regulation, so genes, which expression is activated by it, are of interest as targets for further molecular selection and expression regulation researches. Promoter elements determining gene expression in response to this signal are already known. In the absence of experimental data about certain genes and their products, discovering such elements in their promoters allows to predict promising genes of interest with high probability. In this research, we conducted search for abscisic acid response elements ABRE and CE1 in promoters of genes of Quercus robur and Populus trichocarpa, and potential genes of interest were found.