In-vitro and In-vivo Wound Healing Acceleration by Biogenic Silver Nanoparticles

¹ Department of Pharmacology, School of Pharmacy, RK University, Rajkot -360020, Gujarat, India, M - +91 9913110021, pravin.tirgar@rku.ac.in
² Department of Pharmacology, School of Pharmacy, RK University, Rajkot -360020, Gujarat, India; M - +91 5747 87856, adhiyajinal@gmail.com
³ Department of Microbiology, School of Science, RK University, Rajkot -360020, Gujarat, India, M - +91 99232 68332, sougata.ghosh@rku.ac.in
⁴ Department of Microbiology, School of Science, RK University, Rajkot -360020, Gujarat, India, M - +91 94681 90322, swati.goswami@rku.ac.in

^* Corresponding author: pravin.tirgar@rku.ac.in

Abstract

Aim: In-vivo and In-vitro evaluation of potential biogenically (from plant Dioscorea bulbifera, Dioscorea oppositifolia, Plumbago zeylanica, Gloriosa superba) prepared silver nanoparticles (AgNPs) in wound healing process.

Materials and methods: In-vitro anti-bacterial activity of D. bulbifera, D. oppositifolia, P. zeylanica and G. superba prepared AgNPs in Gram-positive and Gram-negative bacterial pathogens performed using cup and plate assay. In-vitro phagocytic stimulatory assay used to find out potential antibacterial activity of AgNPs. In-vivo pharmacological evaluation of inflammatory potential of topical ointment of D. bulbifera AgNPs using Carrageenan induced paw edema model and In-vivo wound healing property by excision wound model in rats.

Results and Discussion: Biogenic Nanoparticles prepared shows potent anti-bacterial activity towards Gram negative and Gram-positive bacterial strains. Accelerated chemotaxis behavior of neutrophils towards Candida cells observed by concentration dependent increase in % phagocytosis. AgNPs have potency to accelerate chemotaxis of immune cells and found to be anti-microbial in nature. The topical nano-ointment was formed and showed least paw swelling (75.9 + 2.5 %) after 1 hour as compared to other groups in paw edema inflammatory model in albino rats. The significant potentiation in wound healing process observed by rapid closure of wound and reduction in re-epithelization period. Histopathological studies revealed rejuvenation power towards newly formed cells and scarless healing. In- vivo studies revealed that the topical formulation developed from the D. bulbifera prepared AgNPs has successfully accelerated the wound healing process and proven to be good anti-inflammatory agent.