| Issue |
E3S Web Conf.
Volume 655, 2025
International Conference on Chemical and Material Engineering in conjunction with the International Symposium on Applied Chemistry (ICCME-ISAC 2025)
|
|
|---|---|---|
| Article Number | 01020 | |
| Number of page(s) | 6 | |
| Section | Chemical Engineering | |
| DOI | https://doi.org/10.1051/e3sconf/202565501020 | |
| Published online | 27 October 2025 | |
Production and Characterisation of Cellulases from Semi-solid-state Fermentation of Oil Palm Empty Fruit Bunch Using Various Fungi
1 Chemical Engineering Department, Institut Teknologi Bandung (ITB), Jalan Ganesa 10, Bandung 40132, Indonesia
2 Food Engineering Department, Institut Teknologi Bandung, Jalan Let. Jen. Purn. Dr. (HC). Mashudi No.1/Jalan Raya Jatinangor KM 20,75, Sumedang 45363, Indonesia
* Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
In this study, an agricultural waste – oil palm empty fruit bunch (OPEFB) – was used as cellulose source in semi-solid-state fermentation (SSSF). This objective of this research is to produce cellulase using a monoculture SSSF system involving five fungal species: A. niger, T. reesei, T. viride, P. chrysogenum, and R. oryzae. Fermentation was conducted on OPEFB with a solid-to-liquid ratio of 1:20 in 200 mL Erlenmeyer flasks under continuous agitation in an incubator shaker at 35 °C for 9 days. Sampling on days 3, 5, 7, and 9 showed that A. niger attained the optimum fungal cellulase activity of 0.054 FPU/mL. Both T. reesei and T. viride exhibited moderate enzyme activity, while P. chrysogenum and R. oryzae displayed lower activity. HPLC analysis of Whatman No. 1 filter paper hydrolysate identified sugar profile, including glucose at retention time of 9.8 minutes. SDS-PAGE analysis revealed protein bands corresponding to the molecular weights of cellulase complexes and Cellic® CTec2.
© The Authors, published by EDP Sciences, 2025
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