Advancements in Programmable Hydrogels: Synthesis, Characterization, and Their Applications in Medicine

¹ Process Engineering and Environment Laboratory, FSTM, Hassan II University of Casablanca Morocco.
² Université Marie et Louis Pasteur, UTBM, CNRS, institut FEMTO-ST, F-90010 Belfort, France.
³ University Franche-Comté, STAPS Besançon, UMR Right, EPSI, College of Medicine Besançon, 25030, France.
⁴ Laboratory of Useful Materials, INRAP, Ariana 2020 / Faculty of Sciences of Bizerte, Zarzouna, University of Carthage, Bizerte 7021, Tunisia.
⁵ Animal Science Department, High Institute of Agronomy of Chott-Mariem, TN-4042 Sousse, Tunisia.

^* Corresponding author: souad.tayane@univh2c.ma
^† Corresponding author: jaafar.gaber@utbm.fr

Abstract

This article establishes an integrated framework for programmable hydrogels, clarifying design principles, characterization methods, and core uses in healthcare and agriculture while assessing the contribution of nanotechnology. Hydrogels are three-dimensional hydrophilic polymer networks whose properties can be tuned by chemical crosslinking, physically assembled networks, and hybrid strategies— including 3D/4D printing—to control stiffness, toughness, and responsiveness to pH, temperature, light, electric or magnetic fields, and biological cues. Incorporating nanomaterials such as metallic or magnetic nanoparticles, carbon nanostructures, and DNA architectures enhances mechanical strength, conductivity, biocompatibility, and logic-encoded responsiveness, enabling dynamic “smart” behavior. Optimization relies on multiscale characterization combining mechanical testing, thermal analysis (DSC/TGA), imaging (SEM/TEM), atomic force microscopy, and swelling studies to link structure, stability, and morphology with function. Applications include controlled drug delivery, biomimetic scaffolds for adhesion and tissue regeneration, biosensing interfaces, soft robotic actuators, and targeted agro-environmental systems. Advances at the intersection of materials science, nanotechnology, and advanced fabrication point to a new generation of multifunctional hydrogels with strong translational potential, while highlighting remaining challenges in durability, long-term biocompatibility, manufacturing reproducibility, and precise spatiotemporal control of responses. An earlier version of this work was posted as a preprint on ChemRxiv [1].