Isolation And Characterization Of Keratinase-Producing Microbes From Chicken Slaughterhouse Waste Soil With Double Enrichment And Initial Characterization Of Keratinase

Department of Chemistry, State University of Malang, Malang, Indonesia.

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Abstract

Every year, there is an increase in chicken meat consumption. The high consumption of chicken meat results in significant waste from chicken slaughter, including chicken feathers. The soil around the chicken slaughterhouse waste disposal has the potential to produce bacteria capable of producing high proteases and keratinases. This research aims to obtain microbial isolates capable of degrading chicken feather waste and to characterize the microbial isolates and the resulting enzymes. The microbes obtained were enriched twice, namely by adding soil with chicken feathers and using a liquid medium supplemented with chicken feathers. This is done by getting bacteria that are really able to degrade keratin from chicken feathers. From the results of double enrichment, 10 isolates were obtained, with MSF6 being the isolate that produces the highest levels of protease and keratinase enzymes, at 761.532 U/mL and 37.733 U/mL, respectively, at 57 °C and pH 8. Molecular identification using the 16S rRNA sequence, MSF6 can be identified as a Bacillus paramycoides with a resemblance as great 100%. Based on the results of SDS-PAGE and zymography tests, the protease produced from MSF6 has a molecular weight of 68.181 ± 0.068 kDa and 68.147 ± 0.068 kDa, respectively. The enzyme produced from the MSF6 isolate can be used for animal feed, glue, and other industries that require proteases and keratinases.